ILLUMINA (called "sequencer platform" in SRA). The runs can be filtered by a sequencer vendor, e.g.The table can be filtered using the button Filter Settings. Again the issue could be solved by using the 'Append SRA experiment accession to SeqSphere+ Sample ID' checkbox option to assemble the SRA samples separately. Finally, in the unlikely case that two SRA samples have no Strain Name but the same Sample Alias, a warning 'Different SRA samples with the same SeqSphere+ Sample ID were found!' would be shown. In this case either use the the 'Append SRA experiment accession to SeqSphere+ Sample ID' checkbox option to assemble the SRA samples separately or apply the 'SRA samples' filter. Similar, if there would be SRA samples with the same Strain Name also those reads would assemble wrongly together. To avoid this latter case either use the 'Append SRA experiment accession to SeqSphere+ Sample ID' checkbox option to assemble the experiments separately or apply the 'SRA experiments' filter. However, also multiple SRA experiments of the same SRA sample would be assembled together. Downloading FASTQs and metadata with default settings would result in assembling multiple SRA runs of the same SRA experiment together once a pipeline with default file naming parameters would be started. ![]() If there are potential problems with the Sample ID, context-sensitive warnings are shown below the table in the left corner of the window. The SeqSphere+ Sample ID determines how the downloaded reads are treated. In addition the SRA run accession is attached with a leading underscore to the FASTQ File Name Trunk. The underscore and other special characters (e.g., !, :, /) are in the SeqSphere+ Sample ID and the FASTQ File Name Trunk replaced against empty space (the unchanged names are stored in the searchable Strain and Alias ID(s) SeqSphere+ data fields, respectively). If the SRA sample has no Strain Name attached then the Sample Alias or the Sample Title is taken instead. By default the Strain Name is taken as SeqSphere+ Sample ID and the FASTQ File Name Trunk. All SRA samples have a Sample Alias and most SRA samples have a Strain Name and a Sample Title that all must not be unique. A SRA sample can contain multiple SRA experiments and it is usually not a good idea to assemble reads across various experiments. A SRA experiment can contain multiple SRA runs done from the same library. Each SRA run belongs to a SRA experiment, each SRA experiment belongs to a SRA sample, and each SRA sample belongs to a SRA study. ![]() ![]() The found metadata for the given accessions are shown in a table, where each row represents one SRA run. Result table with meta-data for all runs that were found for the given accessions
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